中药胶剂修复半月板损伤的实验研究

目的 观察中药胶剂促进半月板损伤修复的效果,并探讨其作用机理。方法 选用同样条件饲养,体重３ｋｇ左右的健康成年日本大耳白兔８０只,雌雄不拘,每组４０只,均采用左右侧自身对照,侧别由掷币法随机决定。一组动物为空白对照,即一侧半月板裂伤植入中药胶剂,另一侧裂伤不做任何处理;另一组动物为实验对照,即一侧裂伤植入中药胶剂,另一侧裂伤植入物理性状与该中药胶剂相似的明胶。在日本大耳白兔双侧下肢内侧半月板前１／     

正常足与外翻足的足底压力研究

目的　对正常足与外翻足的足底压力进行测量。方法　运用自行研制的足底生物力学测试系统对30 名健康人60 足,45 例外翻患者89 足进行了行走时足底压力的测试。结果　重度外翻组31 足和正常组相比,第一跖骨头下压力明显减低( P< 0.001),第二跖骨头下压力明显增加(P< 0 .05),第三、五跖骨头下压力有增高的趋势。结论　正常人前足第一跖骨头下压力最高,并向外侧递减。重度外翻足前足第一跖骨头下压力明显减低,第二跖骨头下压力明显增高     

神经根型颈椎病(急性期)动物模型的建立

目的：通过模拟颈神经根炎方法,建立神经根型颈椎病的动物模型,方法：将１８０只Ｗｉｓｔａｒ大鼠随机分为三组：模型组,假手术组及正常组。于造模后３天,７天,１４天,２８天,进行病理学观察;３天,７天,１４天,进行Ｐ物质,前列腺素Ｅ２观察;１４天,进行６－酮－前列腺Ｆ１α,血栓素Ｂ２含量及体感诱发电位观察;结果;本模型可以出现明显的炎症,瘀血,水肿及神经功能传导的损伤的变化。结论;模拟颈神经根炎的动物模     

大鼠“曲泽”,“大陵”和“中冲”穴区之间神经血管显微结构的比较

ObjectiveThe aim of this study was to compare the microstructure of blood vessels and nerve fibers between different acupoints in the rat skin from the perspective of histology.MethodsThe skin tissue at “Qūzé” (曲泽 PC3)” “Dàlíng (大陵 PC7)” and “Zhōngchōng (中冲 PC9)” regions was taken from the rat forelimb along the pericardium meridian, and cut sagittally with a freezing microtome. After that, the skin sections were stained by fluorescent histochemistry and immunohistochemistry with phalloidin and calcitonin gene-related peptide (CGRP) to reveal the microstructure of blood vessels and nerve fibers respectively, and then examined under a laser scanning confocal microscope.ResultsThe microstructure of blood vessels and nerve fibers was clearly labeled with phalloidin and CGRP, respectively. The blood vessels and nerve fibers were observed in the dermis of “PC3” “PC7” and “PC9” regions, where the bundle of nerve fibers distribute in parallel to the blood vessels, and sent out thin branches to surround the wall of the blood vessels. In contrast, the density of blood vessels and nerve fibers in “PC3” “PC7” and “PC9” were presented orderly in an increased tendency.ConclusionThe results of present study demonstrate the differences of the neurovascular microstructure among “PC3” “PC7” and “PC9” in the rat, providing a histological view to insight into the specificity of different acupoints.     

交感神经及其递质在椎动脉型颈椎病中的调控机制

目的:验证交感神经因素在椎动脉型颈椎病发病中对椎动脉供血不足的调控机制。方法:将40只家兔随机分为两组:模型组和对照组,每组各20只,在造模当日及造模后4周,分别检测每组的SPNs放电次数及NA的含量。结果:模型组胸髓侧角的SPNs放电次数57.45±7.89,56.60±7.44;对照组胸髓侧角的SPNs放电次数10.08±2.26,10.00±2.24。两组比较,P<0.001。模型组血浆中NA含量(5.48±0.46)pmol/ml,(5.36±0.43)pmol/ml;对照组血浆中NA含量(3.31±0.43)pmol/ml,(3.30±0.32)pmol/ml。两组比较,P<0.001。结论:交感神经因素在椎动脉型颈椎病发病中对椎动脉的供血不足起到重要的调控作用。